polymorphism in the first intron of interferon-gamma gene (+874t/a) in patients with bcg adenitis

نویسندگان

n parvaneh dept. of pediatrics, infectious disease research center, children's medical center, tehran universit

b pourakbari dept. of pediatrics, infectious disease research center, children's medical center, tehran universit

kh daneshjoo dept. of pediatrics, imam khomeini hospital, tehran university of medical sciences, iran

h ashraf dept. of pediatrics, infectious disease research center, children's medical center, tehran universit

چکیده

background: cytokines and specially interferon-gamma (ifn-g) are largely responsible for the regulation of the protective im­mune response against mycobacterial infections. several studies have clarified the importance of common variants of ifn- g gene regarding the susceptibility to tuberculosis. bacille calmette-guérin (bcg) vaccine that is used to prevent se­vere forms of tuberculosis could produce local and systemic side effects. in this study we hypothesized that the ifn- g (+874t/a) polymorphism was associated with development of bcg adenitis. methods: thirty patients with bcg adenitis (18 males and 12 females) and 30 age and sex-matched healthy children, vacci­nated with bcg during the first two days of life were chosen. all the patients and controls were of iranian fars origin and the study was conducted from 2005 to 2007. dna samples were obtained from 30 patients with bcg adenitis and 30 age and sex matched healthy vaccinees. polymorphism at +874 was identified using allele specific polymerase chain reac­tion. allele and genotype frequencies in cases and controls were compared using the χ 2 test and odds ratios (or) and their 95% confidence intervals (ci) were calculated. results: the minor allele (t) frequency was significantly lower in patients with bcg adenitis compared to controls (35% vs. 55%, p = 0.02, or= 0.441, 95% ci= 0.211-0.919). the armitage trend test revealed a gradually increasing protection from the aa genotype through at to tt (common odds ratio= 0.49; p = 0.037). conclusion: our data suggest that in an iranian population, the ifn- g (+874t/a) polymorphism is associated with develop­ment of bcg adenitis in the vaccinees.

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عنوان ژورنال:
iranian journal of public health

جلد ۳۸، شماره ۳، صفحات ۱۲-۱۶

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